Biochemistry
Biochemistry. Instructor: Prof. S. Dasgupta, Department of Chemistry, IIT Kharagpur. This course is an introductory course that will focus on basic concepts in biochemistry. The course deals with an understanding of biological macromolecules: proteins, carbohydrates, lipids, and nucleic acids. The structure and functional roles of the macromolecules will be studied in addition to fundamentals of enzyme chemistry: kinetics, mechanisms, inhibition, structure and mechanism. The course will also touch upon the basics of membrane transport and bioenergetic principles. After completion of the course, the students should be able to understand the chemical properties and three-dimensional structure of these biological macromolecules in relationship to their biological function. (from nptel.ac.in)
| Lecture 12 - Enzyme Mechanisms II |
Concepts covered in this lecture: 1) Catalytic mechanism of serine protease, 2) Other serine protease like Chymotrypsin, Trypsin and Elastase, 3) Amino acid composition determination, 4) Edman degradation, 5) Protein can be specifically cleaved into peptide to facilitate analysis, 6) Chemical reagents and their cleavage site for protein cleavage.
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