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Fundamentals of Optical and Scanning Electron Microscopy

Fundamentals of Optical and Scanning Electron Microscopy. Instructor: Dr. S. Sankaran, Department of Metallurgical and Materials Engineering, IIT Madras. This course will cover the basic principles and techniques of optical and scanning electron microscopy along with demonstrations on the instrument details and imaging experiments through videos. This course also deals with the sample preparation techniques for the microstructural analysis with practical examples through videos. (from nptel.ac.in)

Lecture 16 - Factors Affecting Interaction Volume, Demonstration of SEM

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Fundamentals of Optics

Lecture 01 - Properties of Light, Diffraction and Interference, Theory of Image Formation

Lecture 02 - Magnification and Resolution

Lecture 03 - Depth of Field and Depth of Focus, Contrast, Lens Defects

Lecture 04 - Lens Defects (cont.), Filters, Light Microscopy Introduction

Lecture 05 - Optical Microscope Demo, Bright Field Imaging, Illumination of Opaque Specimen

Microscopy and Sample Preparation

Lecture 06 - Opaque Stop Microscopy, Phase Contrast Microscopy

Lecture 07 - Dark Field Microscopy, Polarization Microscopy

Lecture 08 - Differential Interference Contrast, Fluorescence Microscopy

Lecture 09 - Sample Preparation Techniques for Optical Microscopy

Lecture 10 - Tutorial Problems related to Optical Microscopy

Basics of Scanning Electron Microscopy

Lecture 11 - Introduction to Scanning Electron Microscopy

Lecture 12 - Lens Aberrations, Object Resolution, Image Quality

Lecture 13 - Interaction between Electrons and Sample, Imaging Capabilities, Structural Analysis, Elemental Analysis

Lecture 14 - SEM and its Mode of Operation, Effect of Aperture Size, Effect of Working Distance, Effect of Condenser Lens Strength

Lecture 15 - SEM and its Mode of Operation (cont.), Relation between Probe Current and Probe Diameter, Summary

Lecture 16 - Factors Affecting Interaction Volume, Demonstration of SEM

Lecture 17 - Image Formation and Interpretation

Lecture 18 - Image Formation and Interpretation (cont.), Energy Dispersive Spectroscopy (EDS), Wavelength Dispersive Spectroscopy (WDS)

Lecture 19 - Special Contrast Mechanisms, Monte Carlo Simulations of Interaction Volume

Lecture 20 - Electron Channeling Contrast Imaging (ECCI), Electron Backscattered Diffraction (EBSD) - Theory and Instrument Demonstration

Lecture 21 - Tutorial Problems related to Scanning Electron Microscopy