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Principles and Applications of NMR Spectroscopy

Principles and Applications of NMR Spectroscopy. Instructor: Prof. Hanudatta S. Atreya, NMR Research Centre, IISc Bangalore. The objective of the course is to teach the basic aspects of nuclear magnetic resonance (NMR) spectroscopy, which is an important analytical tool in chemical and pharmaceutical industry for structural characterization of molecules. The topics to be covered will include one-dimensional NMR, Chemical shifts, J-coupling, Interpretation of 1D NMR spectrum, Basics of 2D NMR, Different 2D NMR experiments and their application/interpretation, Application of 2D NMR for assignment of molecules and peptides. (from nptel.ac.in)

Lecture 36 - Chemical Exchange

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Instruction to NMR Spectroscopy

Lecture 01 - Instruction to NMR Spectroscopy

Lecture 02 - The Alignment of Nuclear Spins in Presence of Magnetic Field

Lecture 03 - Introduction to Rotating Frame

Lecture 04 - Free Induction Decay and Fourier Transformation of FID

Lecture 05 - NMR Hardware

Chemical Shifts and J-Coupling

Lecture 06 - The Concept of Chemical Shift

Lecture 07 - Factors that Affect Chemical Shifts

Lecture 08 - Chemical Shift Referencing

Lecture 09 - J-Coupling

Lecture 10 - Recap of Basics

One-Dimensional Proton NMR

Lecture 11 - Introduction to General One-Dimensional NMR Experiment

Lecture 12 - Practical Aspects of Recording a 1D NMR Experiment I

Lecture 13 - Practical Aspects of Recording a 1D NMR Experiment II

Lecture 14 - Practical Aspects of Recording a 1D NMR Experiment III

Lecture 15 - NMR Data Processing

One Dimensional NMR of X-nuclei (13C, 15N, 31P and 19F)

Lecture 16 - Basic Aspects of 1D Proton NMR Analysis

Lecture 17 - Analysis of an Example of 1D Proton Spectrum

Lecture 18 - Analysis of 1D 1H NMR Spectra of Molecules

Lecture 19 - Analysis of 1D 1H NMR Spectra of Molecules (cont.)

Lecture 20 - 1D 13C NMR

Homonuclear 2D NMR

Lecture 21 - Why do We Need 2D NMR?

Lecture 22 - A Qualitative Explanation of How 2D NMR Experiment Works

Lecture 23 - Principles of 2D COSY and Total Correlation Spectroscopy (2D TOCSY)

Lecture 24 - 2D NOE Spectroscopy

Lecture 25 - 2D NOESY and 2D ROESY

Heteronuclear 2D NMR

Lecture 26 - What is Heteronuclear Correlation NMR Spectroscopy?

Lecture 27 - Sensitivity Enhancement of Heternuclei via Polarization Transfer

Lecture 28 - Heteronuclear Multiple Quantum NMR Spectroscopy (2D HMQC) and Heteronuclear Single Quantum NMR Spectroscopy (2D HSQC)

Lecture 29 - Practical Aspects of Recording and Processing 2D HMQC or HSQC

Lecture 30 - HMQC and its Utility

Structure Determination of Molecules

Lecture 31 - 2D HSQC TOCSY and its Analysis with Examples

Lecture 32 - Structure Determination of Molecules by NMR

Lecture 33 - Structure Determination of Peptides I

Lecture 34 - Structure Determination of Peptides II

Lecture 35 - Structure Determination of Peptides III

Advanced Topics (Solvent Suppression, Drug Discovery, DOSY)

Lecture 36 - Chemical Exchange

Lecture 37 - Hydrogen or Deuterium Exchange

Lecture 38 - Diffusion Ordered Spectroscopy (DOSY)

Lecture 39 - Diffusion Ordered Spectroscopy (cont.)

Lecture 40 - STD NMR for Drug Target Interactions