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Eukaryotic Gene Expression: Basics and Benefits

Eukaryotic Gene Expression: Basics and Benefits. Instructor: Prof. P. N. Rangarajan, Department of Biochemistry, IISc Bangalore. The objective of this course is to expose the viewer to basic aspects of regulation of gene expression in eukaryotes and explain the benefits that have accrued out of basic research in this area. The course is organized such that the viewer will learn not only gain knowledge on the molecular mechanisms involved in eukaryotic gene regulation but also will appreciate the benefits that have accrued through the exploitation of knowledge gained from the basic research, primarily in the areas of disease diagnosis and their prevention, agriculture etc. Through this course the instructor wishes to convey the message that knowledge of eukaryotic gene regulation is essential for understanding and appreciating a number of biotechnological applications in the areas of medicine and agriculture. (from nptel.ac.in)

Lecture 19 - Regulation of Gene Expression by Growth Factors


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Cis-acting Elements and Trans-acting Factors
Lecture 01 - Eukaryotic RNA Polymerases and Basal Transcription Factors
Lecture 02 - Diversity in Core Promoter Elements
Lecture 03 - Diversity in General Transcription Factors
Lecture 04 - Proximal and Distal Promoter Elements, Enhancers and Silencers, Gene-Specific Regulators
Domain Structure of Eukaryotic Transcription Factors
Lecture 05 - Eukaryotic Transcription Factors: The DNA Binding Domain
Lecture 06 - Eukaryotic Transcription Factors: Transcription Activation Domains
Eukaryotic Gene Regulation
Lecture 07 - Role of Chromatin in Eukaryotic Gene Regulation
Lecture 08 - Role of Histones in Eukaryotic Gene Regulation
Lecture 09 - Role of DNA Methylation in Eukaryotic Gene Regulation
Lecture 10 - Chromatin Remodelling and Gene Regulation
Synthesis of mRNA, rRNA and tRNA
Lecture 11 - Co-transcriptional and Post-transcriptional Modifications of Pre messenger RNA
Lecture 12 - Co-transcriptional and Post-transcriptional Modifications of Pre messenger RNA
Lecture 13 - Regulation of RNA Pol I Transcription
Lecture 14 - Regulation of tRNA and 5s rRNA Synthesis by RNA Polymerase III
Regulation of Gene Expression via Cell Surface Receptors
Lecture 15 - Signal Transduction Pathways: Introduction
Lecture 16 - Regulation of Gene Expression by cyclic AMP
Lecture 17 - Regulation of Gene Expression by Second Messengers Other Than cAMP
Lecture 18 - Regulation of Gene Expression by Protein Kinase C
Lecture 19 - Regulation of Gene Expression by Growth Factors
Lecture 20 - Regulation of Gene Expression by Cytokines
Regulation of Gene Expression by Intracellular Receptors
Lecture 21 - Regulation of Gene Expression by Steroid Hormones
Lecture 22 - Regulation of Gene Expression by Type II Nuclear Receptors
Lecture 23 - Mechanism of Transcriptional Activation by Nuclear Receptors
Regulation of Gene Expression during Development
Lecture 24 - Gene Regulation during Drosophila Development
Lecture 25 - Signal Transduction Pathways Involved in Embryonic Development
Lecture 26 - Homeotic Genes
Lecture 27 - Epigenetic Regulation of Gene Expression during Development
Lecture 28 - Embryonic Stem Cells and Transcription Factor-Mediated Epigenetic Reprogramming
Recombinant Protein Expression Systems
Lecture 29 - Cloning and Expression Vectors
Lecture 30 - Eukaryotic Protein Expression Systems
Lecture 31 - Eukaryotic Protein Expression Systems (cont.)
Lecture 32 - Eukaryotic Protein Expression Systems: Gene Expression in Mammalian Cells using Viral Vectors
Gene Therapy and Transgenic Technology
Lecture 33 - Human Gene Therapy
Lecture 34 - DNA Vaccines
Lecture 35 - Transgenic Animals
Lecture 36 - Transgenic Plants
Lecture 37 - Knockout Mice
Recent Advances in Eukaryotic Gene Expression
Lecture 38 - Regulation of Eukaryotic Gene Expression by Small RNAs (RNA Interference, RNAi)
Lecture 39 - Genomics and Proteomics
Lecture 40 - Metabolic Engineering and Synthetic Biology