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5.08J Biological Chemistry II

5.08J Biological Chemistry II (Spring 2016, MIT OCW). Instructors: Prof. JoAnne Stubbe and Prof. Elizabeth Nolan. This course is an advanced treatment of biochemical mechanisms that underlie biological processes. Topics include macromolecular machines such as the ribosome, the proteasome, fatty acid synthases as a paradigm for polyketide synthases and nonribosomal polypeptide synthases, and polymerases. Emphasis will be given to the experimental methods used to unravel how these processes fit into the cellular context as well as the coordinated regulation of these processes. (from ocw.mit.edu)

Lecture 01 - Introduction

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Lecture 01 - Introduction

Module 1: Translation

Lecture 02 - Protein Synthesis 1

Recitation 01 - Determining, Analyzing, and Understanding Protein Structures

Lecture 03 - Protein Synthesis 2

Lecture 04 - Protein Synthesis 3

Lecture 05 - Protein Synthesis 4

Recitation 02 - Pre-steady State and Steady-state Kinetic Methods Applied to Translation

Lecture 06 - Protein Synthesis 5

Lecture 07 - Protein Synthesis 6

Module 2: Protein Folding

Lecture 08 - Protein Folding 1

Recitation 03 - Pre-steady State and Steady-state Kinetic Methods Applied to Translation

Lecture 09 - Protein Folding 2

Lecture 10 - Protein Folding 3

Lecture 11 - Protein Folding 4

Recitation 04 - Purification of Native and Mutant Ribosomes, Protein Purification

Module 3: Protein Degradation

Lecture 12 - Protein Degradation 1

Lecture 13 - Protein Degradation 2

Lecture 14 - Protein Degradation 3

Recitation 05 - Overview of Cross-Linking, Including Photoreactive Cross-Linking Methods

Module 4: Polyketide and Nonribosomal Peptide Assembly Lines

Lecture 15 - PK and NRP Syntheses 1

Lecture 16 - PK and NRP Syntheses 2

Recitation 06 - Macromolecular Electron Microscopy Applied to Fatty Acid Synthase

Lecture 17 - PK and NRP Syntheses 3

Lecture 18 - PK and NRP Syntheses 4

Module 5: Cholesterol Biosynthesis and Cholesterol Homeostasis

Recitation 07 - Application of Single Molecule Methods

Lecture 19 - Cholesterol Biosynthesis 1

Lecture 20 - Cholesterol Biosynthesis 2

Lecture 21 - Cholesterol Biosynthesis 3 and Cholesterol Homeostasis 1

Recitation 08 - Application of CRISPR to Study Cholesterol Regulation

Lecture 22 - Cholesterol Homeostasis 2

Lecture 23 - Cholesterol Homeostasis 3

Lecture 24 - Cholesterol Homeostasis 4

Recitation 09 - Cholesterol Homeostasis and Sensing

Lecture 25 - Cholesterol Homeostasis 5 and Metal Ion Homeostasis 1

Module 6: Metal Ion Homeostasis

Lecture 26 - Metal Ion Homeostasis 2

Lecture 27 - Metal Ion Homeostasis 3

Recitation 10 - Metal-Binding Studies and Dissociation Constant Determination

Lecture 28 - Metal Ion Homeostasis 4

Lecture 29 - Metal Ion Homeostasis 5

Recitation 11 - Mass Spectrometry

Lecture 30 - Metal Ion Homeostasis 6

Lecture 31 - Metal Ion Homeostasis 7 and Reactive Oxygen Species 1

Module 7: Reactive Oxygen Species (ROS)

Recitation 12 - Mass Spectrometry of the Cysteine Proteome

Lecture 32 - Reactive Oxygen Species 2

Lecture 33 - Reactive Oxygen Species 3

Lecture 34 - Reactive Oxygen Species 4 and Nucleotide Metabolism 1

Module 8: Nucleotide Metabolism

Recitation 13 - Fluorescence Methods

Lecture 35 - Nucleotide Metabolism 2

Lecture 36 - Nucleotide Metabolism 3