Principles of Downstream Techniques in Bioprocess (Prof. Mukesh Doble, IIT Madras) | Biology and Biological Engineering

Principles of Downstream Techniques in Bioprocess

Principles of Downstream Techniques in Bioprocess. Instructor: Prof. Mukesh Doble, Department of Biotechnology, IIT Madras. A product that is manufactured in a bioreactor or a fermentor, is recovered and purified in several subsequent unit operations. The economy of a manufacturing process is determined by the cost effectiveness of these downstream operations. This course discusses these operations and the basic underlying principles with worked out problems. (from nptel.ac.in)

Introduction

Lecture 01 - Introduction

Lecture 02 - Mass Balance, Heat Balance, Flow Sheet

Lecture 03 - Costing

Lecture 04 - Cell Breakage

Lecture 05 - Solid Liquid Separation

Lecture 06 - Pre-treatment and Filters/Centrifuges

Lecture 07 - Liquid-Liquid Extraction

Lecture 08 - Liquid-Liquid Extraction (cont.)

Lecture 09 - Adsorption

Lecture 10 - Reverse Micellar and Aqueous Two Phase Extraction

Lecture 11 - Membranes

Lecture 12 - Membranes (cont.)

Lecture 13 - Product Stabilization, Drying, Lyophilisation

Lecture 14 - Precipitation and Crystallisation

Lecture 15 - Electrophoresis

Lecture 16 - Chromatography 1

Lecture 17 - Chromatography 2

Lecture 18 - Chromatography 3

Lecture 19 - Chromatography 4

Lecture 20 - Future Trends, Other Downstream Operations

References

Principles of Downstream Techniques in Bioprocess
Instructor: Prof. Mukesh Doble, Department of Biotechnology, IIT Madras. A product that is manufactured in a bioreactor or a fermentor, is recovered and purified in several subsequent unit operations.