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Principles of Downstream Techniques in Bioprocess

Principles of Downstream Techniques in Bioprocess. Instructor: Prof. Mukesh Doble, Department of Biotechnology, IIT Madras. A product that is manufactured in a bioreactor or a fermentor, is recovered and purified in several subsequent unit operations. The economy of a manufacturing process is determined by the cost effectiveness of these downstream operations. This course discusses these operations and the basic underlying principles with worked out problems. (from nptel.ac.in)

Lecture 15 - Electrophoresis


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Lecture 01 - Introduction
Lecture 02 - Mass Balance, Heat Balance, Flow Sheet
Lecture 03 - Costing
Lecture 04 - Cell Breakage
Lecture 05 - Solid Liquid Separation
Lecture 06 - Pre-treatment and Filters/Centrifuges
Lecture 07 - Liquid-Liquid Extraction
Lecture 08 - Liquid-Liquid Extraction (cont.)
Lecture 09 - Adsorption
Lecture 10 - Reverse Micellar and Aqueous Two Phase Extraction
Lecture 11 - Membranes
Lecture 12 - Membranes (cont.)
Lecture 13 - Product Stabilization, Drying, Lyophilisation
Lecture 14 - Precipitation and Crystallisation
Lecture 15 - Electrophoresis
Lecture 16 - Chromatography 1
Lecture 17 - Chromatography 2
Lecture 18 - Chromatography 3
Lecture 19 - Chromatography 4
Lecture 20 - Future Trends, Other Downstream Operations