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Analytical Technologies in Biotechnology

Analytical Technologies in Biotechnology. Instructor: Dr. Ashwani K. Sharma, Department of Biotechnology, IIT Roorkee. Welcome to this course on analytical technologies in biotechnologies. It is quite evident that advances in technology has been vital in the progress of science. In life sciences, advances in analytical techniques have played a crucial role in understanding of biological functions at the molecular and the cellular label. Biotechnology is an interdisciplinary science that involves applications of chemical and physical techniques, in the field of life sciences. This course covers lessons on Microscopy, Radioisotope techniques, Chromatographic methods, Electrophoresis, Centrifugation techniques, Spectroscopic techniques, Polymerase Chain Reaction(PCR), DNA sequencing, and Enzyme linked immunosorbent assay. (from nptel.ac.in)

Lecture 18 - Gel Filtration Chromatography

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Microscopy

Lecture 01 - Basic Concepts in Microscopy

Lecture 02 - Basic Concepts in Microscopy (cont.)

Lecture 03 - Dark-field and Phase Contrast Microscopy

Lecture 04 - Differential Interference Contrast and Polarization Microscopy

Lecture 05 - Fluorescence and Confocal Microscopy

Lecture 06 - Transmission Electron Microscopy

Lecture 07 - Transmission Electron Microscopy (cont.), Scanning Electron Microscopy

Radioisotopes Techniques

Lecture 08 - Basic Concepts

Lecture 09 - Basic Concepts (cont.)

Lecture 10 - GM Counting and Scintillation Counting

Lecture 11 - Scintillation Counting (cont.)

Lecture 12 - Autoradiography and RIA

Lecture 13 - Safety Aspects and Applications

Chromatographic Methods

Lecture 14 - Introduction and Basic Concepts in Chromatography

Lecture 15 - Basic Concepts in Chromatography

Lecture 16 - Low-Pressure Liquid Chromatography (LPLC) and High Performance Liquid Chromatography (HPLC)

Lecture 17 - Ion-exchange Chromatography

Lecture 18 - Gel Filtration Chromatography

Lecture 19 - Affinity Chromatography

Lecture 20 - Gas-Liquid Chromatography

Electrophoresis

Lecture 21 - Basis Concept in Electrophoresis

Lecture 22 - Horizontal and Vertical Gel Electrophoresis

Lecture 23 - Native Gel Electrophoresis and SDS-PAGE

Lecture 24 - Isoelectric Focusing, 2D Gel Electrophoresis and Protein Detection Methods

Lecture 25 - Electrophoresis of Nucleic Acids

Lecture 26 - Immunoelectrophoresis and Capillary Electrophoresis

Centrifugation Techniques

Lecture 27 - Introduction and Basic Concepts

Lecture 28 - Basic Concepts (cont.)

Lecture 29 - Types of Centrifuges and Analytical Ultracentrifugation Method

Lecture 30 - Separation Methods in Preparative Ultracentrifuges

Lecture 31 - Types of Rotors

Lecture 32 - Types of Rotors (cont.), Care of Rotors

Spectroscopic Techniques

Lecture 33 - Introduction and Basic Concepts

Lecture 34 - UV-Visible Spectroscopy

Lecture 35 - Infrared and Fluorescence Spectroscopy

Lecture 36 - Circular Dichroism (CD) Spectroscopy

Lecture 37 - Nuclear Magnetic Resonance (NMR) Spectroscopy and X-ray Crystallography

Lecture 38 - Atomic Spectroscopy and Mass Spectrometry

Polymerase Chain Reaction

Lecture 39 - Polymerase Chain Reaction (PCR)

Lecture 40 - DNA Sequencing Methods

Lecture 41 - Enzyme Linked Immunosorbent Assay (ELISA)