7.01SC Fundamentals of Biology (Fall 2011, MIT OCW). Instructors: Prof. Eric Lander, Prof. Graham Walker, Prof. Sallie Chisholm, et al. This consists of a series of video lectures given by Prof. Eric Lander, Prof. Robert Weinberg, Prof. Tyler Jacks, et al.. This course focuses on the basic principles of biochemistry, molecular biology, genetics, and recombinant DNA. These principles are necessary to understanding the basic mechanisms of life and anchor the biological knowledge that is required to understand many of the challenges in everyday life, from human health and disease to loss of biodiversity and environmental quality.
(from ocw.mit.edu)
Lecture 20 - cDNA Libraries and Expression Libraries
References
Lecture 20 - cDNA Libraries and Expression Libraries
This session will review how to make a recombinant cDNA library and how to use this library to find a specific gene. This session will outline the differences between a genomic and a cDNA library, and discuss how to use a cDNA library to clone a gene of interest.